Abstract
A totalof seventy five water samples were collected from three different levels;level 1(surface), level 2 (sub-surface), and level 3(bottom) of a fish pond and subjected to enumeration and isolation of colony forming units of coliform bacteria by growing on EMB agar. Highest number of CFUs/ml was obtained in the samples collected from level 3. Following pure culturing, each isolate was processed for colonial characteristics, biochemical characterization and staining reactions. These isolates exhibited gamma and beta hemolysis but none of them was found resistant against polymyxin B contrary to erythromycin. These isolates were identified as Klebsiella and Citrobacterand the latter genus dominant.

Shagufta Andleeb, Maria Maryam , Aqsa Aslam. (2014) Quantitative analysis of a fish pond for coliform bacterial content, Punjab University Journal of Zoology, Volume 29, Issue 1.
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