Abstract
Monovalent copper is determined in different herbs and their mixture using differential pulse polarography (DPP) and standard
addition method is employed. Selected herbs are Nigella sativa (Kalonji), Trigonella foenum-graceum (Fenugreek) and Linseeds
(Alsi), in the form of mixture they are taken in ratio 2:1:1 respectively. For the extraction of samples aqueous as well as acidic media
were used. In the acidic medium the average values of concentrations for individual herbs and also in the mixture are greater as
compare to the aqueous medium. The average value of copper in the mixture is found to be moderate in both media as compare to the
individual herbs. These values also comparable to the reported tolerance limit. Polarograms were recorded in the differential pulse
mode with multimode electrode system of Metrohm A. G. model 746 VA Trace analyzer connected with data based software. Copper
is required to the body in very little amount but this little quantity is very important and essential. The quantitation is made by standard
addition method and linearity is calculated by linear regression line. Linearity of differential pulse polarography is checked by the
calibration curves.
