Mutations in the muscle proteins can have variable affects in the body. If the mutation is in the constitutive exon all the muscles will be affected whereas if it is in the non-constitutive one than effects will be localized to the area where the protein from that exon is involved. To observe and analyze the effects of the mutation by statistical methods is one of the most powerful tools to highlight the degree of the effect. Here we are reporting in detail the behavioural effects of a muscle Troponin-T gene mutation known as up1 and its comparison to that of the wild-type flies.