Sugarcane probably represents the most complex genome being studied todate. Current cultivars are derived from complex interspecific hybridization and they are highly polyloid and aneuploid with 100 to 130 chromosomes. When cells having such high aneuploid and plant plantlets regenerated from these cells their genetic fidelity become under question. Therefore, RADP (Random amplified polymorphic DNA) technique was used to detect variations in the directly regenerated plants of sugarcane. Plants directly regenerated from tissue of NIA-98 were studied for existing genetic variability with in the tissue in the form of aneuploidy. The polymorphism were observed in 39 out of 42 bands (92.8%). Polymorhism of band confirms that direct regeneration cannot maintain genetic fidelity in sugarcane but can be considered as a good source of exploring existing aneuploidy.
Imtiaz A. Khan, M. U. Dahot, Sajida Bibi, Saboohi Raza. (2007) GENETIC INSTABILITY OF SUGARCANE PLANTS REGENERATED THROUGH DIRECT REGENERATION METHOD, , Volume 4, Issue 1.
