Abstract
Iron is determined in the seeds of Nigella sativa spectrophotometrically and iron-opt complexation is selected. Calibration curve method is employed for this study. From this curve molar absorptivity (ε) was calculated to determine the concentration of metal in different samples, molar absorptivity (ε) was found to be 10,000M-1 . cm-1 at 515nm. Average values of concentrations for the five ´amples were found to be 110.4239ppm, 139.7700ppm, 138.0647ppm, 141.6273ppm and 139.2660ppm.respectively. Fe(III) solution was treated with extract of N. sativa as well as two other active biological reductants, hydroquinone and hydroxyl ammonium chloride. It has been found that N. sativa is stronger reducing agent than hydroxyl ammonium chloride and weaker then hydroquinone.
