A soil bacterium (CMG500) capable of degrading a fungicide buprimate (Nimrod), was isolated from vegetable fields and identified as Achromobacter species. It harbors a catabolic plasmid pNS3. The isolate was resistant to streptomycin, penicillin, and ceferozone. The structure of the degraded product was elucidated using mass spectra, U. V. and N. M. R. It was found that the fungicide bupirimate (5-Butyl – 2-ethyl amino 6-methyl-4pyrimidyl dimethyl sulfamate was converted into 5- Butyl-2 ethyl amino 6-methyl –4 –pyrimidinol by Achromobacter species. Hydrolysis of buprimate fungicide resulted in the removal of dimethyl sulfamate group. The degrading genes for buprimate were present on a conjugative plasmid pNS3.
A. Nuzhat, A. Jameela, R. Munawar, Atta-ur-Rahman. (2005) HYDROLYSIS OF A FUNGICIDE, BUPRIMATE BY INDIGENOUS ACHROMOBACTER SPECIES, , Volume 2, Issue 2.
