Abstract
Fusariutn solani, a mesophilic fungus, was grown for 4 days at 35°C, pH 5 under solid state growth conditions using wheat bran for the production of amyloglucosidase.The specific activity of crude enzyme was 23.08 units me.The enzyme was purified using fractional precipitation, anion-exchange and gel filtration chromatography. The recovery of amyloglucosidase after gel filtration was 35.65 % with 6.32 fold increase in specific activity. Maximum activity of the enzyme was observed at 40°C and pH 5.0. The Km value for soluble starch was 0.4 mg m1-1. Amyloglucosidase from F.solani was thermally stable as it possessed a half-life of about 30 min at 56°C
