Cell culture-derived soluble Anaplasma marginate vaccine admixedwith a saponin adjuvant was administered in single dose, 2 weeks between doses to 6 months rats. An additional 10 rats served as non-vaccinated controls. Comparable groups of these rats were challenge exposed at 90 and 100 days after vaccination, using 1X 105 A. marginate organisms contained in freshly collected blood from a splenectomized rats with ascending parasitemia. Challenge exposure led to no deaths of vaccinated rats, whereas 5 of the control (non-vaccinated) rats died by A. rnrginale. All clinical and hematologic variables examined indicated that the vaccinated rats had gained immunologic protection in comparison with the control group. An immune recognition as manifested by an anamnestic humoral response to challenge exposure was in evidence in the vaccinated rats.
M. J. Haider, N. Arain and N. Qureshi. (2005) CELL CULTURE-DERIVED ANAPLASMA MARGINALE VACCINE : SEQUENTAL CHALLENGE EXPOSURE OF PROTECTIVE IMMUNITY DURING A 3-MONTH POST VACCINATION PERIOD, , Volume 2, Issue 4.
