Abstract
The immature ooctyes have long been used for experimental trials for betterment of production of genetically superior breed both in
terms of meet and milk production. In these experiments immature oocyte were harvested from the buffalo (Bubalus bubalis) and
cattle ovaries (obtained from slaughter house) and transported to the laboratory at 28 oC in phosphate buffer saline solution (PBS)
within two hours of slaughtering. The ovaries were sliced with a ten unit blade slicer in PBS supplemented with 0.4% bovine serum
albumin (BSA) and 250 iu/L heparin. Good quality oocytes were incubated in four different media i.e. TCM199, Ham’s F10, MEM,
and IVF Universal medium (medicult) supplemented with 10 iu/ml follicle stimulating hormone (FSH), 10 iu/ml Leutinizing hormone
(LH) and 1.0 µg/ml estradiol 17-β. The average maturation rates for cattle and buffalo oocytes were 56.0+1.9 % and 55.4+0.8 %
respectively. There was no significant difference in maturation rates of oocytes among these media. The immature vitrified oocytes
were incubated after thawing at 37 oC for maturation rates. No difference could be detected in the maturation rates between cattle
(52.2+0.13 %) and buffalo oocytes (47.85+0.2 %). The maturation rates of vitrified COCs were significantly lower after vitrification
than untreated COCs, both in cattle and buffalo.
