Abstract
Shoot tip and nodal segment explants were cultured on MS medium modified with different growth regulators for mass propagation of 'Hybrid Tea' roses cvs. 'Rosy Cheek' and 'Whisk Mac'. The Murashige and Skoog medium modified with BAP (3 rnql,") + IAA (3 rnql,") + activated charcoal (5 rnql,') effectively minimized production of phenolic compounds and gave early shoot proliferation, maximum shoot length and number of leaves per shoot. Nodal segments gave earlier sprouting, higher shoot initiation percentage and longer shoot length compared to shoot tip explants. Proliferated shoots were transferred to different combinations of root initiation media and roots were only regenerated in MS medium supplemented with 0.5 rnql," IBA after 19 days of subculturing. Key words: Rose, in vitro, explants, browning control, mass propagation, shoot initiation, root regeneration
