Abstract
Different explants excised from reproductive organs of papaya (Carica papaya L.) were appraised on half strength Murashige and Skoog (MS) medium. It was supplemented with 2,4-D (2.5. 5.0 & 7:5 rngl.") and kinetin (0.5, 1.0 & 1.5 mgl.") for callogenesis. separately. Highest initial callus induction (100% and 75%) was found in ovary explants on Kinetin (1.5 and 0.5 mg L") in first week of culture, respectively. Ovary and embryo proved to be the best explant for callus induction (68.57% and 67.1%) and callus growth (68.72 mg and 62.7 mg), respectively. Use of these two as explant source might be suggested as the best among various reproductive parts explored. Kinetin proved better growth hormone for both callus induction and callus growth than 2,4-D. This protocol can be helpful to propagate male and female plants swiftly by subsequent embryogenesis and organogenesis. It may further be contributive in developing synthetic seeds and transgenic plants of papaya. Key words: Callogenesis; Papaya; In Vitro; 2,4-D; Kinetin; reproductive explants; embryo; nucellus
