Abstract
Reproductive explant derived callus of'papaya (Carica papaya L) was subcultured on Murashige and Skoog (MS) media supplemented with different levels of NAA for embryogenesis and subsequent organogenesis, Embryo and ovary derived callus yielded maximum multiplication (80%) on NAA 5 and 3 mgL'1 in first week. Ovary (80%) derived callus multiplied at the highest rate while NAA 5 rngl." proved to be better treatment than others, Embryogenesis started on NAA 3 mgL I in first week of subculture and was highest (40%) on NAA 1 mgl." and control. Organogenesis initiated on both NAA I mg L· 1 and control in fifth week and was better on control (30%), Only embryo derived callus developed somatic embryos and subsequent root and shoots, The protocol might be promising to propagate female plants swiftly. developing synthetic seeds and transgenic plants of papaya. Key words: Callus, embryogenesis. papaya. in vitro, NAA, reproductive explants, embryo
