Abstract
The present study was designed to determine the matched estradiol-17B concentration in serum, fore-milk and saliva during different phases of buffalo oestrous cycle by radioimmunoassay technique. Estradiol- 17B concentration in all experimental biological fluids was higher during pro-oestrus followed by oestrus, dioestrus and met-oestrus. There was a close positive correlation between the salivary estradiol-17B concentration (r=:=0.92),fore-milk (r=0.87) and serum samples (r=0.88). It was concluded that saliva can be used to determine estradiol-L'ZBconcentration as an alternate of milk and serum. Key words: buffaloes, estradiol-17B, fore-milk, oestrous cycle, saliva, serum
