Abstract
A protease producing fungal strain was isolated from a sludge soil collected from Lahore. It was cultured on Potato dextrose agar (PDA)
plates at 37 º C mended with 1 % casein. The strain, identified as
Aspergillus niger, produced maximum zone of clearance and was selected for further studies. Different parameters such as different carbon sources, different ratios of carbon and nitrogen sources; incubation period, inoculum size, % moisture temperature and pH, were investigated in solid state fermentations. Maximum enzyme production was obtained at 8:2 of carbon and nitrogen (wheat bran: soybean) sources after 48 hours incubation with 5 ml inoculum size at 30 º
C and 3.0 pH. After optimizing these parameters, ultraviolet mutagenesis of this strain was attempted at 254 nm. Eight mutant strains were isolated for the maximum protease production. These strains were observed under optimized conditions for the maximum production of proteases. The strain 90S1 was found as best protease producer. Partial characterization of proteases and their stability in the absence and presence of CaCl2 were determined.
JAVAID IQBAL QAZI , HUFSA JAMSHAID, MHAMMAD NADEEM , SYED SHAHID ALI. (2008) PRODUCTION OF PROTEASES BY ASPERGILLUS NIGER, THROUGH SOLID STATE FERMENTATION, Punjab University Journal of Zoology, Volume 23, Issue (1&2).
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