Abstract
Pentosan polysulphate (PPS) is highly negatively charged polysaccharide compound and is significant inhibitors of
Guanidinobenzoatase (GB), associated with tumour cells in the sputum
spreads of lung cancer patients, and free GB in solution. Texas red
labeled PPS (TR-PPS) selectively recognized and binds the GB
associated with the tumour cell surfaces, and these cells fluoresced
slightly black-red. 9-aminoacridine (9-AA) is another low molecular
weight yellow fluorescent inhibitor of GB and can recognize and bind
with both isozymic forms of GB, associated with tumour and normal
tissues. If the sputum spreads are stained with 9-AA, they behave
similarly as with TR-PPS in recognizing and binding with this probe and
fluoresce brightly yellow under the fluorescent microscope. Kinetic
studies have shown that PPS inhibit the fee GB in solution in a
concentration-dependent manner. These studies suggest that TR-PPS is a potent inhibitor of tumour cells GB and can be used as a novel fluorescent probe for the location of tumour cells in the sputum spreads of the patients suffering from lung cancers.
MUHAMMAD ANEES, AKSA JAWAD. (2008) LOCATION OF THE TUMOUR CELLS IN THE SPUTUM SPREADS WITH FLUORESCENT PROBE PENTOSAN POLYSULPHATE , Punjab University Journal of Zoology, Volume 23, Issue (1&2).
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