Abstract
Crude ethanolic extract of Nerium oleander leaves was evaluated for its insecticidal and antibacterial activities. Stored grain pest, Trogoderma granarium and fruit fly Drosophila rufa larvae were used as insect model. The leave extract was prepared in ethanol. The recovered
dried extract was 9.4g/100gram of fresh leaves. Dried extract was
suspended in ethanol for exposing T. granarium and D. rufa. To check
the contact effect of the crude extract, 2nd and 4th instar larvae of T.
granarium and 3rd instar larvae of D. rufa were used. Three methods viz.., filter paper, residual film and feeding were used. Concentration of N. oleander extract used were 10, 25, 50 and 100mg. T. granarium larvae showed 10% mortality after 72 hours at 100mg dose level. In D. rufa 10% mortality occurred after 48 hours and 15% after 72 hours at 20mg dose level. Antimicrobial activity of N. oleander crude extract was evaluated by disc diffusion method against both Gram-positive and –negative bacterial strains. Nine doses of crude extract i.e., 0.4, 0.5, 0.7, 0.8, 0.9, 1.00, 1.2 and 1.4 mg/disc for each strain. Four strains i.e., Bacillus cereus, cellulose producing Bacillus, B. alves and Acinetobacter anitratus showed growth inhibition zones at all above concentrations.
SYED SHAHID ALI , SAMINA ALI, SHAHID MUNIR , TANZEELA RIAZ. (2008) INSECTICIDAL AND BACTERICIDAL EFFECTS OF ETHANOLIC LEAF EXTRACT OF COMMON OLEANDER, NERIUM OLEANDER, Punjab University Journal of Zoology, Volume 23, Issue (1&2).
-
Views
423 -
Downloads
41
