Abstract
The study reports the bacterial profiles of four locations of the canal. Mud of the given localities were sampled, when the water supply was closed. Bottom soils of canal were sampled in sterile containers, from four different locations in the urban area. In the laboratory, l0gm of a soil sample was suspended in 100ml of autoclaved water and processed for; enumeration of colony forming units (CFU) representing endospore
formers on nutrient agar plates, and cellulose degrading bacteria on a selective agar medium. While for the coliforms MacConkcy agar was used. Pure cultures of coliforms and endospore formers were processed for determining their antibiotics and heavy metal sensitivity/resistance. Too many CFUs of cellulose degrading bacteria were obtained for
the first three samples. These bacterial populations appeared to degrade the sediment cellulose material and useful for providing relatively less turbid and of low biological oxygen demand water to the downstream areas. For the fourth sampling locality CFU of cellulose degrading bacteria reduced drastically, which indicated the lesser amounts of the available substrate (cellulose material) at this place, downstream. Hemolytic endospore formers were isolated from all samples. Fecal contamination of the canal bed became evident from the presence of coliform bacteria in all the samples. It is concluded that different types of bacteria are inhabitants of the canal bed, performing their ecological roles. The bed is also a good indicator, as assessed by the presence of hemolytic endospore formers and coliform bacteria of pathogen contamination and fecal pollution of the habitat.
SAJIDA AMIN, NAUREEN CHAUDHRY , JAVED IQBAL QAZI. (2005) CELLULOLYTIC, ENDOSPORE FORMER AND COLIFORM BACTERIAL PROFILES IN THE BOTTOM MUD OF LAHORE CANAL AT DIFFERENT LOCATIONS IN THE CITY , Punjab University Journal of Zoology, Volume 20, Issue 2.
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