Abstract
Sixty two Agrohacteriunt tumefaciens strains were isolated from local plants of Punjab, Pakistan. Their metallic salts and antibiotics resistance/sensitivity profiles, growth response at two different temperatures and at two different temperatures with various p1-1 levels were investigated. The results. of these experiments revealed that isolates shared many physiological characters. Majority of strains could tolerate different metallic salts but all of them could grow in the presence of MnSO4, PbNO3 and ZnSO4 and all were sensitive to Cr salt. These strains could be divided into 15 groups on the basis of metallic salts resistance/ sensitivity profiles. While for different antibiotics these strains showed different behaviour and could he grouped into 10 -clusters on the basis of their antibiotics resistance/sensitivity profiles. Results of bacterial growth experiments at 28° and 37°C and at different levels of pH (4. 5. 6. 7. 8, 9) were almost similar in some aspects. Most of the strains, initially, showed better growth rate at 37°C except some strains isolated from colder areas which exhibited better growth at 28°C. The experiments performed at different levels of pH revealed that all strains showed maximum growth rate at pH 6. However pH affect on the growth rate exhibited that all strains could grew better in extreme alkaline pH (9) as compared to extreme acid pH (4).
S.H. AMER QAZI , SHAHIDA HASNAIN. (2000.) INDIGENOUS AGROBACTERIUM TUMEFACIENS: GROWTH RESPONSES TO METALLIC SALTS, ANTIBIOTICS, TEMPERATURE AND pH, Punjab University Journal of Zoology, Volume 15, Issue .
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