Growth medium of milk whey for the production of lactic acid by Lactobacillus acidophilus was developed and different conditions were optimized for lactic acid production. Maximum yield of lactic acid (4.4 %) was recovered from shake medium in the presence 01'0.1 % NaCI. 0.1 % MgS04.7Hp. 0.1 % NH4H2P04, 2% molasses. 0.1 % yeast extract and 2.0% corn steep liquor after 48 hours incubation at 35 C and pH 5.5 on orbital shaker.
