Abstract
Of the five Neovossia indica inoculation methods, boot inoculation with by syringe proved to be the best, followed by drop inoculation method of clipped florets, dip inoculation of unclipped florets and spray inoculation of heads, in that order, Screening of 80 wheat gerniplasni lines revealed 3 lines to be immune, 8 moderately resistant, 10 moderately susceptible, 21 susceptible and 38 highly susceptible.
INTRODUCTION
Artificial inoculation of crop germpla.sm with the inocula of their pathogens is a usual procedure for screening out sources of resistance against these pathogens. However, the successful screening depends upon the successful in fections which, in turn, depend upon the inoculation technique used. Thus various techni-ques for the inoculation of wheat germplasm with Neovossia indica (Mitra) Mundkar4 the cause of karnal bunt disease, were evaluated to know the most reliable and dependable inoculation method. Later by the use of this method, wheat gerrnplasm was inoculated and screening for the sources of resistance against the pathogen was carried out.
* Plant Pathologist, Wheat Research Institute, Faisalabad.
