Abstract
Chickpea {Ckcr ari'airiim L.) var. +51h3 nodes were cultured on supplemented B-5 medium, The combination or auxin (3 mg NAA} and cytoltinin O. .mg BAP) gale cani of approrpiate degree of britireoess and fresh weight.. These caIli yielded more (9 x ]1.12) free single cells in suspension as compared to compact calli(2x102),
INTRODUCTION
The riecessiry of exoenously supplied auxin for r vitro production of calkus from an explant suss reported. by Earle and Torrey (1965). Explants from almost any part of the plant, such as seeds, items, roots. IleiKS or fruits can develop a callus by culturing on the surface of the medium (Street, 1977). Continued sub-culture of those callus masses can continue for long periods of time (Earle, 1974; Hartmann. and Koster, 1983).
Although callus tissue culture may appear to be a uniform mass of cells, but in reality its structure is relatively complex with considerable morphological, physio'rogioal and genetic variation. within the callus (Street,. 1977; Sarwar, 1984.) A number of different culture media has been used various research workers to grow call.us i e„ MS, Rlivr, Sid, etc. These media contain macroele-meats, particularly nitrogen as nitrate (NO3) and arrimcniurn ions (HH1),, sucrose and certain 'vitamins but initiation of cell division and callus production require both a cytokiniii and an auxin 5uPplied in the proper proportion (Skoog and Miner, 1957), Auxin, as IAA, NAA or 2,41.-D is the hormone primarily used at a high concentration to produce callus. Cytokinin as kitetin or i5.benzyla.minopurine (BA?) is supplied in true' arnovnt on the basis of signi-ficant effect of auxin and cytokinin on carcus dr\ e!oprr.cnt (Eatirrann and Kester, 1983).
in the presen.
