Abstract
Callus masses were developed from pith parenchyma tissue and cut edges of the leaves of sugarcane on _modified MS medium supplemented with 1-5 mg 1 of 2, 4-D. Response to callus initiation was different in various varieties and 2, 4-D concentrations. Callus differentiated to produce numerous shoots on modified MS medium adapted with kinetin and casein hydrolysate. Shoots on transfer to medium devoid of growth regulators induced rooting. Plantlets so formed (I800 in number) were grown in pots in the glasshouse and later transferred to the field. Structures comparable to embryoes were observed in callus cultures grown on low concentration of 2,4-D. Further incubation of these cultures on the same medium or medium without growth regulators, produced rooted plantlets. Morphological variants were observed in clones derived from the callus of each variety.
