Abstract
Clinical facility, for the present study, was available at the Institute of Nuclear Medicines and Oncology (INMOL) and Sheikh Zayed Hospital (SZH), Lahore. Serum samples of healthy women and hypothyroid patients were obtained along with the radioimmunbassay values of triiodothyronine (T3), thyroxine (T4) and thyroid stimulating hormone (TSH). Samples were diluted in phosphate buffer (pH 7.2) and proteins were denatured by heating with loading dye. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), based on the method of Laemmli (1970), was employed for studying the protein profile of healthy and hypothyroid subjects. Gels were photographed and their images were stored for quantification of various protein fractions by UVP Gel Base Software Programme that provides the data of molecular weights and percent areas covered by each of the fractions. Data was employed in finding the enhancement or reduction and the appearance or disappearance of particular protein fractions for comparison between healthy and hypothyroid groups. Thirty-eight fractions were detected in normal whereas 49 were detected in hypothyroid subjects. Most of the fractions in hypothyroid group were foUnd to be significantly enhanced when compared to healthy group. Fractions of molecular weights 156 and 26 kDa, detected in healthy group, could not be expressed in hypothyroid group, however, thirteen new fractions of molecular weights 325, 123, 91, 68, 65, 62, 60, 55, 50, 40, 31, 17 and 12 kDa were found to appear in hypothyroid group which could not be detected in healthy group.
NABILA ROOHI, ABDUL MAJEED CHEEMA, AYESHA FATEH MUHAMMAD, MUHAMMAD WAHEED AKHTAR. (1999) ELECTROPHORETIC ANALYSIS OF SERUM PROTEIN FRACTIONS IN HYPOTHYROID WOMEN , Punjab University Journal of Zoology, Volume 14, Issue 1.
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