Abstract
An organochlorine insecticide, lindane (gamma-HCH) was administered to three groups of Sprague Dawley rats. @ 30 mg/kg body wt. once, 18 mg/kg body | wt./day and 9 mg/kg body wt./day, for 48 hours, 15 days and 18 months, respectively. Following stipulated durations, animals were dissected, their livers were removed, fixed quickly and processed further by routine histological techniques. Liver sections, 6-8 pm
- thick were cut, stained with haematoxylin and eosin technique and examined under | : microscope for morphometric and pathological changes. Lindane treatmént produced significant increase (10-59%) in hepatic cell size in all experiments. The nuclear and : nucleolar size also showed similar increase with 26% and 73% maximum change. The
number of cells/microscopic field decreased by 18%, 22% and 26% in 48 hour, 15 days and 18 month experiments. No significant change was found in number of nuclei/cell and number of nucleoli/nucleus. The liver sections revealed moderate to high cellular pathology in different treatments. Cellular and nuclear hypertrophy was evident in-almost all treatments. The clear areas in the cytoplasm showed proportionate increase with increase in duration of insecticide teatment. Increased sinusoidal spaces, condensed/hyperchromatic and distorted nucle), fatty changes, increase in kupffer cells and clear areas around the nuclei were the other prominent changes.
SYED SHAHID ALI , ABDUL RAUF SHAKOORI. (1998) STUDIES ON THE TOXICITY OF LINDANE IN ALBINO RAT: HISTOPATHOLOGICAL EFFECTS IN LIVER, Punjab University Journal of Zoology, Volume 13, Issue 1.
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