Home Journal List Punjab University Journal of Zoology Volume 5 Article Details

TOXICOLOGY OF ALDRIN IN RATS

Abstract

Aldrin-mixed diet, fed to Sprague Dawley rats at a dose of 20 nig, 8 mg and 2.5mg/kg body wt./day for 48 hours, 15 days, and 18 months, respectively produced significant changes in the blood and liver. The haemoglobin content, red blood cell count and packed cell volume decreased, while the white blood cell count increased under all treatments. The serum glutamate oxaloazetate transaminase (GOT), alkaline phosphatase (AP) and isocivate dehydrogenase increased 370, 141 and 135%, respectively in 48 hour treattne The creatin phosphokinase activity increased (83%) in 15 day treatment. The acid phosphatase activity increased 97% after 6 months treatment. Bilirubin content increased in 15 day, urea in 15 day and 18 month and protein content under all experimental conditions. Cholesterol and free amino acids (FAA) contents decreased after aldrin feeding. In 48 hour experiment hyperglycemia was induced, while hypoglycemia was found in 15 day treatmant. In liver, AP and GOT activities increased 171 % and 28% respectively in 48 hour feeding, while lactic dehydrogenase (LDH) decreased (26%). In 15 day feeding experiment the glutamate pyruvate transaminase (GPT) and LDH, activities increased 186% and 23% after 9 and 15 days, respectively. This increase was mom pronounced in 18 months treatment. The hepatic cholesterol generally decreased in 48 hour and 15 day treatments. The FAA and glucose contents decreased in 48 hour and 6 month treatments, while in 15 day feeding reverse pattern was obtained. Hepatic soluble proteins increased in 48 hour and 18 month feeding groups, DNA content remained unaltered, while RNA showed almost constant increase in 15 day treatment. Aldrin treatment produced hypertrophy of hepatic cells, nuclei and nucleoli. Fatty degeneration and extensive vacuolation was evident in 48 hour feeding. The vacuolation, granulation of hepatic tissue and prominent clear areas around nuclei were found in 15 day feeding. These changes, with distorted nuclei and enlarged kupffer cells, were also observed in 18 months feeding group.

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