Abstract
Objective: To assess the hepatoprotective effects of
Ivy leaf extract in order to overcome the hepatotoxicity
induced by isoniazid in rabbits.
Methodology: This analytical experimental study was
carried out at animal house in the Department of
Pharmacology, Karachi University. The duration of the
study was 60 days. A total of 30 healthy rabbits were
divided randomly into three equal groups. Group I or
control group, group II isoniazid (INH) group while in
group III combined effects of isoniazid and Ivy leaf
extract. Serum alanine transaminase (ALT) level was
measured. ANOVA test followed by post hoc multiple
comparison was applied to assess the difference
between the groups.
Results: There was significant difference in the mean
level of ALT at Day 7 between the groups (p = 0.001).
A significant difference was observed between the
groups at day 30 and 60 (p < 0.001). Multiple
comparisons of ALT level at day 60 showed
significant increase between group I and group II (p <
0.001), significant difference was observed between
group I and group III (p = 0.042), whereas significant
decreased was observed between group II and group
III (p < 0.001). Multiple comparisons of total bilirubin
level at Day 60 in different groups revealed
significantly increased level between group I and II
(p < 0.001) as well as between I and III (p = 0.012)
and between group II and III (p = 0.002).
Conclusion: The oral administration of Ivy leaf
extract produced a hepatoprotective effect in isoniazid
induced liver injury by reducing the serum glutamate
oxaloacetate transaminase level.
Keywords: Hepatotoxicity, Isoniazid, serum alanine
transaminase, hepatoprotective effect
