Abstract
Objective:To study the prevalence of inducible clindamycin along with vancomycin and methicillin resistanceand assessment of hyper variable region (HVR) of mecA gene among different clinical isolates of Staphylococcus spp.Methods:A total of 176 clinical isolates of Staphylococci were collected from Pakistan Institute of Medical Sciences (PIMS), Islamabad during 2014-2015. The sample sources were pus, blood, urine, sputum, tracheal secretions and tissue fluids. Bacterial identification was done by colony morphology and biochemical tests. Kirby-Bauer disc-diffusion method was carried out to assess the susceptibility against different antibiotics. Minimal inhibitory concentrations (MICs) were done for vancomycin resistance. Double Disk Diffusion test (D-test) was used to detect the clindamycin inducible resistance. PCR was performed to detect erm(C), mecA and HVRgenes.Results:Clindamycin inducible resistance among Staphylococcalisolates was found to be 7%, whereas in S. aureus it was 4%, and in coagulase negative Staphylococci (CoNS)it was 11%. The highest resistance was observed against fosfomycin, fusidic acid and cefoxitin. Vancomycin resistance was observed in 23 isolates (13%) of Staphylococci. erm(C), mecA and HVR genes were found in 18%, 50% and42% respectively.Conclusions:D-test must be performed routinely to avoid clindamycin failure. A high level of resistance against vancomycin in Staphylococcal isolates is a concern for public health.KEYWORDS:Clindamycin inducible resistance, D-test, HVR region, mecA, Staphylococci.doi: https://doi.org/10.12669/pjms.36.2.665How to cite this:Khan AA, Farooq J, Abid M, Zahra R. Assessment of inducible clindamycin resistance and Hyper Variable Region (HVR) of mecA gene in clinical staphylococci. Pak J Med Sci. 2020;36(2):136-140. doi: https://doi.org/10.12669/pjms.36.2.665This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Amir Afzal Khan, Jahanzaib Farooq, Madiha Abid, Rabaab Zahra. (2020) Assessment of inducible clindamycin resistance and Hyper Variable Region (HVR) of mecA gene in clinical staphylococci, Pakistan Journal of Medical Sciences, Volume -36, Issue 2.
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