• Yasir Mehmood

• Ikram Ullah Khan

• Yasser Shahzad

• Syed Haroon Khalid

• Sajid Asghar

• Abid Mehmood Yousaf

• Talib Hussain

• Muhammad Irfan

• Ikrima Khalid

Abstract

A simple, specific, accurate and economic reverse phase liquid chromatographic method wasdeveloped forthe estimation of Sofosbuvir in all polymorphic form. Mobile phase containsacetonitrile and 0.05M ammonium acetate (pH 7.6) in ratio of 50:50 (v/v). A logarithmiccalibration curve was plotted from10μgmL-1to60μgmL-1(r= 0.9989) for sofosbuvir, withthelimit of detection (LOD) and the limit of quantification(LOQ)of 0.245μgmL-1and 0.816μgmL-1,respectively. The developed method was highly validated andpracticed for the assurance ofsofosbuvir raw materials with acceptable and non-objectionable accuracy and precision results(recovery 99–102%, RSD <1.2%, n = 3).Zero significant difference (t-test) was obtained betweenthe overall results of the developed RP-HPLC method

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