Survey has highlighted the significance of hand washing and also indicated the knowledge and practices among 250 mothers between the age group 22-35 in belong to Karachi, the cosmopolitan city of Pakistan by direct filling of questionnaire and short interviews. The results of the survey indicated that 24% were also not aware that gastroenteritis and diaherral diseases can be transmitted to their kids due to their improper hand washing practices. Similarly, about 6% were reported of washing hands frequently each day and 72% spend hardly less than a minute in this activity. In addition to this, among interviewed mothers 60% responded washing hands with soap as compared to the 32% and 40% wash with some detergents and plain water respectively. Moreover, 56% mothers were found to have long nails and about 40% of them had dirt in them. In interview, 16% were found only educating their kids about hand washing procedures and 72% of the mothers were in favour of introducing a course of hand hygiene for school going kids