Abstract
Antifungal potential of biochar produced from wood was evaluated
against Fusarium oxysporum Schlecht. Methanol was used to prepare
biochar extract of which various concentrations were experienced in-vitro
against tested fungal strain. All the applied concentrations of methanol
extract i.e. 1%, 2%, 3%, 4%, 5%, 6%, 7% and 8% effectively inhibited F.
oxysporum growth. However, 7% and 8% concentrations were proved to
be most efficient in retarding the test fungus growth upto 15%.
Partitioning of biochar methanolic extract was done using some organic
solvents such as n-hexane, chloroform, ethyl acetate and n-butanol in
increasing the polarity order. These secluded fractions were further tested
to evaluate their in vitro antifungal activity along with a reference fungicide
(Mancozeb). Data obtained after 7 days of incubation depicts that
maximum antifungal activity was observed in fungicide with 89% inhibition
in the radial diameter of the test fungus. n-hexane and chloroform also
effectively retarded the growth of F. oxysporum, whereas n-butanol and
ethyl acetate fractions gave minimum activity . So it can be concluded
that wood biochar has the potential to control F. oxysporum
MAHRUKH SHAHID, KHAJISTA JABEEN, SUMERA IQBAL. (2018) Evaluation of antifungal potential of Wood Biochar against Fusarium oxysporum Schlecht, Biologia – Journal of Biological Society of Pakistan, Volume 64 (II), Volume 64 (II).
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