Abstract
Laccase enzyme has a range of biotechnological and industrial applications
but its high cost hindered its use. Development of low-cost biotechnological
process for laccase production has gained great attention. Present study is
concerned with the enhanced production of laccase by Aspergillus flavus Maf
0139. Five different cultural media were tested and best one was selected for
laccase production. Different cultural conditions such as inoculum size, pH,
temperature, incubation period, moisture content and inducer were studied.
Medium components were further optimized by Plackett-Burman and central
composite designs. Results showed that maximum enzyme activity (297±0.1
U/ml/min) was obtained by using AFM02 medium containing g/L: sawdust, 5.0;
glucose, 5.0; peptone, 6.0; MgSO4.7H2O, 0.25; KCl, 0.5; KH2PO4, 0.025.
Optimized conditions for maximum enzyme yield were inoculum size 2.5%,
Temperature 25˚C, pH 4.5, incubation period 7 days and moisture content
60%. Eleven different inducers were evaluated but FeSO4.7H2O showed
maximum enzyme production (2156±0.57 U/mL/min). By using these
statistical techniques more than 13 folds increase in enzyme yield (4022±0.25
U/mL/min) was observed. It is concluded that cultural conditions have great
influence on enzyme production and enzyme yield could be enhanced by
using advanced statistical techniques.
TAYYABA ASIM, IRFANA MARYAM. (2018) Optimization of cultural conditions for enhanced production of laccase by Aspergillus flavus Maf 0139, Biologia – Journal of Biological Society of Pakistan, Volume 64 (II), Volume 64 (II).
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