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Biotic and abiotic factors badly affect the productivity and growth of plants and found the major yield-limiting factor in agriculture. In recent years, various cis-regulatory elements have been identified that can function as molecular switches to regulate the expression of stress-related genes. They comprise of insulators, silencers, promoters and enhancers. These elements mediate not only functional diversity, but also modulate plant physiology at all developmental stages. Thus, identifying and characterizing cis elements that are intricated in plant stress response is essential for the development of plants tolerance to stress. This review article presents characteristic properties of cisacting regulatory elements. Two aspects of cis-elements are targeted; one is properties and examples of condition-specific cis-elements while the other is techniques used for their identification. This review will be helpful in elucidating recent advancements in cis-elements studies.

Usman Ijaz, Tehreem Pervaiz, Temoor Ahmed, Riffat Seemab, Muhammad Shahid, Muhammad Noman, Majid Nadeem, Farrukh Azeem. (2020) Plant Cis-regulatory elements: methods of identification and applications, Asian Journal of Agriculture and Biology, Volume 8, Issue 2.
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