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Background:
Diabetes and hypertension frequently coexist, leading to additive increases in the risk of life-threatening cardiovascular events. Large scale proteomic studies implicate the role of aberrant protein expression in these groups’ specifically post translational modifications. Protein S-nitrosylation conveys a large part of the ubiquitous effect on cellular signal transduction, accumulating evidence indicates important roles in normal physiology. Dysregulated S-nitrosylation has been implicated as a cause or consequence of a broad range of diseases, including asthma, cystic fibrosis, Parkinson disease, heart failure, and stroke. The purpose of study is to identify molecular changes and potential alterations in expression of specific aberrant s-nitrosylation in hypertensive and diabetic hypertensive patients. We aimed to identify such blood biomarkers and potential drug targets which can provide insight into the underlying molecular mechanisms, associated with its pathology.
Methods:
Proteome mapping of hypertensive, diabetic hypertensive serum samples was conducted to get the expression of aberrant nitrosylated proteins. Serum samples (n=15 from each group) by using sodium dodecyl sulphate polyacrylamide gel electrophoresis coupled with immunoblot by using anti S-nitrosylated antibody followed by imaging and statistical analysis by Quantity-One software (BioRad).
Results:
We have identified in total fifteen nitrosylated protein components with altered expression among the studied groups. The 177.8KDa, 119KDa, 74.02KDa, 61.5KDa, 52.3KDa protein, and 24.93KDa proteins are showing hyper-nitrosylation in diabetic hypertensive serum samples. However, he 119KDa, 74.02KDa and 61.5KDa protein components showed hyper-nitrosylation in hypertensive serum samples as compared to normal controls while rest of the proteins component were found hypo-nitrosylated.
Conclusion:
The characterization of aberrantly expressed nitrosylated proteins globally and their association with disease associated pathways probably are playing modulatory roles in the pathophysiology of the disease, following post-translational modifications.
Erum Zafar, Ayesha Khan, Beena Hasan, Meraj Zehra, Nikhat Ahmed. (2016) Aberrant Protein S-Nitrosylation ;A New Perspective In Hypertensive And Diabetic Hypertensive Disorder, The Pakistan Journal of Medicine and Dentistry, Volume-5, Issue-4.
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