• Muhammad Ashfaq

• Ansa Afzal

• Muhammad Arshad Javed

• Muhammad Ali

• Bilal Rasool

• Shabnum Shaheen

• Muhammad Shafiq

• Urooj Mubashar

• Mubarak Ali Anjum

• Abdul Rasheed

تلخیص

Rice grain morphology is affected by discoloration disease that responsible for causing huge yield loss every year and its threat increasing significantly. Disease scores and identification of pathogen provides the information to control this disease timely. Disease scoring was done at the maturity stage of the crop to classify the rice lines on the basis of disease severity. Different diseased leaf samples were collected in the year 2018 from different rice varieties grown in the research fields of Punjab University, Pakistan to identify the pathogen. From the collected diseased samples, the casual organisms Bacillus cereus from collected rice samples was isolated, purified and identified on the basis of various morphological characteristics and molecular analysis. For this purpose, The DNA was used as a template to amplify the 16S rRNA gene by means of primers 27F 5' (AGA GTT TGA TCM TGG CTC AG) 3 and 1492R 5' (TAC GGY TAC CTT GTT ACG ACT T) 3' (fD1 50-AGAGTTTGATCCTGGCTCAG-30 and rD1 50- AAGGAGGTGATCCAGCC-30) for isolation and identification of causal bacterial species from total genomic DNA. On morphological basis the pathogen Bacillus cereus was re-isolated and reconfirmed from the artificially inoculated rice plant leaves through Koch’s postulates. To our information Bacillus cereus was reported first time in Pakistan and majorly causing grain discoloration disease in rice. For controlling and management of this disease, the current study will be very useful for research scientific and farmers community.

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