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Life cycle stages of plants reflect the environment to which they are subjected to varied factors including abiotic and biotic. They are often exposed to various heavy metals throughout their life span. Certain metals support their growth (usually in small amounts) whereas sometime they suppress their growth pattern. Present study examines the effect of one of the heavy metals i.e. Nickel on seed germination and early seedling growth of Lens culinaris L.at different concentrations. In addition, amylase activity and chlorophyll content of Lens culinaris was investigated during germination and seedling development. Germination data at various time intervals was analysed using Repeated Measures Design instead of commonly but incorrectly used Analysis of Variance (ANOVA) as the former is the appropriate procedure when the successive observations are dependent rather being random and independent. The results reveal that different concentrations of Ni, particularly at higher concentrations (200 and 400ppm) effectively decreased the final germination percentage, speed of germination, photosynthetic pigments (in cotyledons and leaves) and amylase activity of germinating seeds, whereas it slightly increased the amylase activity at 50 and 100 ppm. These findings are discussed in the light of metabolism during germinating seeds and early seedling growth.
S. Shahid Shaukat, Moazzam A. Khan, M. Saad Akhtarand Nageen Tayyab. (2021) EFFECT OF NICKEL ON GERMINATION, SEEDLING GROWTH, CHLOLOROPHYLL CONTENT AND AMYLASE ACTIVITY OF LENS CULINARIS L.:AN APPLICATION OF APPROPRIATE TOOL FOR ANALYSIS OF GERMINATION DATA, , Volume 18, Issue 3.
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