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Fungal diseases are a constant threat to crop production worldwide. For chemical control of fungal diseases fungicides are used excessively which not only pollute the environment but also harm the human health. The increasing demand of production and regulations on the use of agrochemicals and the emergence of pathogens resistant to the products used, justifies the search for novel active molecules and new control strategies. Recent studies have shown that the transgenic approach is useful to control different fungal pathogens. A transgenic potato line was developed using barley Chitinase gene to control fungal pathogens. The transgenic potato AK-22 line was assessed under different biotic and abiotic factors i.e. fungal pathogens, saline environment and heavy metal stress. Herein, we tested the resistance of barley chitinase gene in potato to confers resistance against Fusarium oxysporum and Alternaria solani and found that the expression of barley chitinase gene enhanced with the time after infection in real time PCR amplification. The line found resistant against Fusarium oxysporum, Alternaria solani as well as tolerant against salinity and heavy metal stress.

Mubarak Ali Anjum, Muhammad Ashfaq, Muhammad Saleem Haider, Muhammad Arshad Javed, Muhammad Ali, Bilal Rasool, Muhammad Shafiq, Urooj Mubashar. (2021) BARLEY CHITINASE GENE CONFERS RESISTANCE AGAINST FUSARIUM OXYSPORUM AND ALTERNARIA SOLANI IN TRANSGENIC POTATO AK-22, , Volume 18, Issue 4.
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