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Effect of nutrient media, temperature, hydrogen ion concentration and photoperiod were studied under in vitro conditions to record the maximum colony growth and spore count. Maximum growth was recorded on 28oC on PDA media having pH 6.5 with 16/8 light and dark period. The maximum growth on PDA was 87 mm and spore count was 4.36 × 106 spores/mL while the lowest growth was observed in MEA media which was 47mm and spore was counted as 1.48 × 106 spores/mL. The present studies were found useful to prepare mass inoculum and also by maintain physiological factors attack of Alternaria porri can be decreased in controlled environment.

Justina Jane Tahira, Salik Nawaz Khan , Maroof Siddique. (2019) IN VITRO GROWTH OF ALTERNARIA PORRI, THE CAUSAL AGENT OF PURPLE BLOTCH OF ONION, , Volume 16, Issue 2.
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