• Muhammad Ashfaq

• Urooj Mubashar

• Muhammad Saleem Haider

• Muhammad Sajjad

• Mubarak Ali Anjum

• Muhammad Shahzad

• Muhammad Ali

تلخیص

The study was carried out to check the efficacy of different bio pesticides at equal concentration against Cnaphalocrocis medinalis (Guenee) (Lepidoptera: Pyralidae). In this study DNA quantification was used as pesticide screening and evaluating tool for plant genome health. Different botanicals crude extracts i.e. Azadirachta indica, Melia azedarach, Eucalyptus globulus and one microbial insecticide Bt was used in this experiment for control of rice leaf folder under Randomized Complete Block Design (RCBD) with 3 replications. The neem crude extract and microbial insecticides (Bt) gave excellent results with lowest post treated increase in leaf infestation (2.76, 3.07, 6.60% and 4.48, 6.06, 9.33 % after 3, 9 and 16 days of application, respectively) caused by Cnaphalocrocis medinalis. On the other hand, Melia azedarach and Eucalyptus also controlled the Cnaphalocrocis medinals but with least efficacy. Maximum leaf folder infestation was observed in control plots. Different bio pesticides were used instead of chemical pesticides that harmful to the plant (cause genotoxicity) and checked it may bond or not with plant genome. Rice samples treated with different bio pesticides along with control were collected and extracted the DNA by using CTAB method. All the samples were run on gel electrophoresis for further confirmation and quantification DNA using spectrophotometer. The bio pesticide treated rice samples showed DNA quantification value i.e. 34.45 µg/mL, 33.65 µg/mL, 31.05 µg/mL, 34.55 µg/mL, 33.88 for control, Bt, Eucalyptus, Darek and Neem, respectively. The main objective of the study was to check the genotoxicity effect of bio pesticide treated rice and concluded that no harmful effect of bio pesticides on rice plant structure and cause no genotoxicity of plant genome.

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