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Thirty one strains of Aspergillus niger were isolated using a new approach which describes the selective isolation of A. niger using tannic acid medium. Twelve strains, selected after qualitative screening using Bromocresol green dye, were further screened quantitatively for citric acid production by submerged fermentation. Of all the strains, A. niger GCBT-7 gave the maximum citric acid production (8.27±0.95 g/l). The optimal production of citric acid by the isolate GCBT-7 (11.91+0.76.23 g/l) was achieved 168 h after the conidial inoculation, in the fermentation medium containing 150-g/l sugar. The initial pH of the medium was also optimized and a pH of 5.5 was found to be the best for citric acid production.
Ikram-ul-Haq, Uzma Hameed, Hamid Mukhtar and M. A. Qadeer. (2006) ISOLATION OF CITRIC ACID PRODUCING STRAINS OF ASPERGILLUS NIGER USING TANNIC ACID MEDIUM AND PRODUCTION PARAMETERS, , Volume 3, Issue 4.
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