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Random amplified polymorphic DNA (RAPD) technique was applied to analyze the genetic diversity among six female date palm cultivars (Asseel, Fusli, Khupra, Kurblane, Punjmail, Pathri) and one male. Young leaves of these cultivars were collected from Khairpur. Seventeen arbitrary primers were used for this study. Among these primers, only 9 revealed polymorphic and unambiguously scorable bands. A total of 75 bands were generated with an average of 8.33 bands/primer. Out of 75 amplified fragments, 65 (86.66%) were polymorphic. Cluster analysis by unweighted paired group method of arithmetic mean (UPGMA) showed two groups Group ‘A’ and Group ‘B’. Group ‘A’ consists of Khupra, Kurblane l, Fusli and Pathri while Group ‘B’ consists of Asseel and Punjmai. Maximum genetic similarity (77%) was present between Khupra and Kurblane while minimum genetic similarity (58%) was present between Asseel and Punjmail. Cultivar male did not belong to any group. It was 48% genetically similar to the rest of 6 cultivars. This study may be helpful to know the genetic difference present among local date palm cultivars cultivated in the province of Sindh.
Ghulam Raza, Saboohi Raza, Imtiaz Ahmad Khan and Abdullah Khatri. (2006) GENETIC DIVERSITY ANALYSIS OF LOCAL DATE PALM (PHOENIX DACTYLIFERA L.) CULTIVARS OF PAKISTAN THROUGH MOLECULAR MARKER (RAPD) , , Volume 3, Issue 4.
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