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Forty three strains were isolated from metal contaminated sites close to the residential areas of Karachi, Pakistan. These strains were
found to tolerate heavy metals and antibiotics. In case of heavy metals 62.8% of the strains have shown tolerance against chromate and
copper, and 51.2% against nickel. Eight antibiotics were checked, 97.7% of the strains have shown tolerance against streptomycin,
44.2% against neomycin, 27.9% against ampicillin, 25.6% against tetracycline, 13.9% against chloramphenicol, 9.3% of the strains
have shown tolerance against kanamycin and novobiocin, and 2.3% against rifampicin. Attempt was made to find out correlation
between heavy metal tolerance and antibiotic tolerance of these strains with the assumption of multiple stress tolerance of bacteria.
Statistical analysis has shown positive correlation between heavy metal tolerance and antibiotic tolerance of these strains, t-test has
been conducted to test the null hypothesis of no correlation which has been rejected at t.05 level of significance. Omegon-transposon
containing kanamycin tolerance gene did not hybridize with the strain CMG2K8 which has shown tolerance upto 1500 µg/ml of
kanamycin, when colony hybridization was performed through DIG labelled probe, this result has indicated that mechanism of
tolerance to kanamycin of the strain CMG2K8 is different from the one found in Omegon-transposon.
Erum Shoeb and Nuzhat Ahmed . (2006) CORRELATION OF MULTIPLE STRESS TOLERANCE IN INDIGENOUS BACTERIA, , Volume 3, Issue 1.
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