Abstract
Pesticides have been continuously used by farmers worldwide, including Thailand
leading to their accumulation in agricultural soils. Bioremediation using
microorganisms to degrade pesticides in soils has currently become of interest because
it is considered to be cost effective and safe. The main objectives of this study were to
isolate and characterize bacteria degrading chlorpyrifos, an organophosphate pesticide
commonly used in Thailand. The characterization of the isolated bacteria included
chlorpyrifos tolerance study. The most chlorpyrifos tolerant isolate was further
subjected to the determination of a gene responsible for chlorpyrifos degradation and
molecular identification. For the isolation of chlorpyrifos degrading bacteria from soils,
M9 minimal medium supplemented with 100 ppm was used. Of all 6 isolated
chlorpyrifos degrading bacteria, the most chlorpyrifos tolerant bacterial isolate was a
gram negative bacterial isolate CHL3. It was tolerant to chlorpyrifos as high as 2,000
ppm. From plasmid isolation, it was found that the bacterial isolate CHL3 had no
plasmid, indicating that a gene responsible for chlorpyrifos degradation was on the
chromosome. When it was subjected to the 16S rDNA sequence analysis, it was
identified as Pseudomonas stutzeri. The bacterium obtained from this study might be
useful for its application in bioremediation of agricultural soils contaminated with
chlorpyrifos and organophosphate pesticides.
Parichat Phumkhachorn, Pongsak Rattanachaikunsopon. (2020) Chlorpyrifos degrading Pseudomonas stutzeri isolated from pesticide contaminated soil, Asian Journal of Agriculture and Biology, Volume 8, Issue 3.
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