Abstract
Objective: To study the hepatoprotective effect of Tamarixdioica at low and high doses on transaminases
(Alanine aminotransferase and Aspartate aminotransferase) in acetaminophen induced hepatotoxicity in male
mice.
Study Design:It was a Randomized Control Trial.
Place and Duration of Study: The study was conducted at department of Pharmacology, Islamic International
st st Medical College, Rawalpindi from 1 April 2015 to 31 March 2016.
Materials and Methods: Forty Balb-c Albino male mice were randomly divided in four groups with 10 mice
each. Group A was the control group and received no medications. In Group B (Disease Control Group)
hepatotoxicity was induced by Acetaminophen 1000mg per kg body weight given daily for 4 weeks. Group C
(Low Dose Experimental Group) was given Acetaminophen 1000 mg/kg/day orally in combination with
Tamarixdioica (Aqueous Extract) that was given daily through gavage tube in a dose of 100mg/kg/day for 4
weeks. Group D (High Dose Experimental Group) followed the same protocol as Group C but the Tamarixdioica
(Aqueous Extract) dose was increased to 200mg/kg/day for 4 weeks through gavage tube. ALT and AST were
measured and compared in different groups to see the hepatoprotective effect of Tamirixdioica.
Results: Mean ALT in Group A, B and C were 35.50 2.24 U/L, 94.00 8.62 U/L and 50.90 4.56 U/L respectively.
There was a significant difference among ALT values of Group A & B and among Group B & C (p Value .000).
Mean AST in Group A, B and C were 27.00 3.11 U/L, 151.00 14.53 U/L and 66.90 7.77 U/L respectively. There
was a significant difference among AST values of Group A & B and among Group B & C (p Value .000). This
suggested that hepatotoxicity was induced by acetaminophen and hepatotoxicity was improved by
Tamirixdioica. No significant difference was observed in ALT & AST values among Group C and D.
Conclusion: Acetaminophen induces hepatotoxicity at high dose. Concomitant treatment with aqueous extract
of roots of Tamarixdioica prevents hepatotoxicity induced by Acetaminophen in mice. Extract of roots of
Tamarixdioica showed improvement biochemically in both low and high doses, as compared to drug treated
group in a dose independent manner.
Uzma Riaz, Noman Sadiq, Muhammad Tahir, Noor Nasir Rajpoot, Amanat Ali. (2018) Hepatoprotective Effect of Tamarixdioica Roots on Acetaminophen Induced Hepatotoxicity in Male Mice, Journal of Islamic International Medical College, Volume-13, Issue-4.
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