Abstract
Objective: To study the protective role of omega 3 fatty acids on the histomorphological changes in the area of
the prostatic acini in rats, induced by sleep deprivation.
Study Design: Lab based randomized control trial.
Place and Duration of Study: The study was conducted at Anatomy Department, Army Medical College,
Rawalpindi, in collaboration with National Institute of Health (NIH), Rawalpindi for a duration of one year from
th th 11 Nov 2014 to 11 Nov 2015.
Materials and Methods: Thirty male Sprague Dawley rats, 3-4 months of age with average weights of 200-300
grams (gm) were divided in three groups each having 10 rats .Group A served as control with standard lab diet
and regular sleep -wake cycle. Group B was subjected to sleep deprivation of 16 hours followed by a sleep
window of 8 hrs daily for 2 months and group C was administrated with omega 3 fatty acids and was sleep
deprived as group B for 2 months. At the end of the experimental period rats were anesthetized and their blood
sample was drawn for hormonal assay. They were dissected and the prostate gland was removed and fixed in 10
percent formalin. Five micrometer sections were obtained after tissue processing and stained with
haematoxylin and eosin (H&E) for histological study.
Results: Microscopic examination revealed that the percentage area of the prostatic acini was decreased by
100% in group B and 30% in group C. This revealed that the area of the prostatic acini was decreased in the
group B as compared to the control group A. Decrease in the acinar area in the experimental group C was not
that marked as compared to experimental group B.
Conclusion: It is concluded that sleep deprivation has deleterious effects on the area of the prostatic acini and
that omega 3 fatty acids has an ameliorative effect on the area of the prostatic acini.
Nomana Mahmood Syed, Kaukab Anjum Qureshi, Humaira Ali. (2017) Effects of Sleep Deprivation on the Area of the Prostatic Acini in Rats and the Protective Effects of Omega 3 Fatty Acids, Journal of Islamic International Medical College, Volume-12, Issue-3.
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