Abstract
Objective: To determine the protective role of Pomegranate Juice (PJ) and Pomegranate Peel Extract (PPE) on
steroid induced serum creatinine level in mice.
Study Design: Randomized control trial.
Place and Duration of Study: Department of Anatomy, Army Medical College Rawalpindi, in collaboration with
st th the National Institute of Health (NIH), Islamabad, from May 1 to June 30 , 2015.
Materials and Methods: Forty healthy mice (BALB/c strain), weighing 25-30 gms on average were randomly
divided into four groups, each group having five male and female mice. Group A was control group and groups
B, C and D were experimental groups. Mice in experimental groups were injected Nandrolone decanoate, 1
mg/100 gm body weight, intramuscularly in the hind limb once a week for 8 weeks. Mice in experimental group
C were also given pomegranate Juice daily, 3ml/kg body weight by oral gavage tube for 8 weeks. Mice in
experimental group D were given Pomegranate Peel Extract daily, 200mg/kg bodyweight through oral gavage
tube for 8 weeks. After eight weeks 5ml blood was drawn from mice through cardiac puncture just before the
sacrifice of animals. Data were entered and analyzed by using SPSS version 21. ANOVA test was applied for
intergroup comparison of quantitative variables. P-value of <0.05 was taken as significant.
Results: Serum creatinine level was significantly higher in experimental group B. It was improved statistically
when compared with pomegranate juice and pomegranate peel extract administered experimental groups C
and D, respectively. When results of experimental groups C and D were compared with each other no statistical
significance was present.
Conclusion: Both forms of pomegranate equally ameliorate the steroid induced effects on serum creatinine
level in mice.
Humaira Ali, Nomana Mahmood, Gulshan Trali, Khadija Qamar, Zarmina Saga. (2017) Protective Role of Pomegranate Juice and its Peel Extract on Steroid Induced Raised Serum Creatinine in Mice, Journal of Islamic International Medical College, Volume-12, Issue-1.
-
Views
934 -
Downloads
92
Article Details
Volume
Issue
Type
Language
