Abstract
Objective: To determine the effect of caffeine on the weight and length of femur of BALB/c mice.
Study Design: A Laboratory based randomized control trial.
Place and duration of study: The study was conducted at Anatomy Department, Army Medical College,
th Rawalpindi in collaboration with National Institute of Health, Islamabad for a duration of one year from 6
th October 2014 to 5 October 2015.
Materials and Methods: Twenty BALB/c mice (10 male, 10 female) , three weeks old, weighing 12-14 g, were
taken and divided into two groups with 10 mice (5 male, 5 female) in each group. The control group G1 was
given normal diet and water ad libitum. Each animal in the experimental group G2 was given 10mg of caffeine
per 100g body weight on alternate day, three days in a week by oral gavage for 60 days. The effect of caffeine
was evaluated by measuring the weight and length of femur of the BALB/c mice at the end of study. IBM-SPSS
version 20 was used for data analysis. The student's T-test was applied for intergroup comparison of
quantitative variable, which was taken as means and standard deviations (mean ± SD). A p value < 0.05 was
taken as significant.
Results: The mean femur weight of BALB/c mice of control group G1 was observed as 0.387±0.019 g while the
mean femur weight of experimental group G2 was found to be 0.316±0.020 g. However, the mean femur length
of control group G1 was 20.70±0.609 mm and experimental group G2 was 24.382±1.087mm. The weight of
femur was decreased in experimental group G2 while the length of femur was increased in experimental group
G2 as compared to control group G1.
Conclusion:Caffeine consumption causes reduction in femur weight and increase in femur length.
Maimoona Khan, Shadab Ahmed Butt. (2017) The Effect of Caffeine on the Weight and Length of Femur of Balb/C Mice, Journal of Islamic International Medical College, Volume-12, Issue-1.
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