Abstract
Objective: To determine the Reno-protective effect of ethanolic extract of Cassia fistula (Amaltas) leaves on
streptozotocin induced diabetic nephropathy in rats.
Study Design:An animal experimental study
Place of Study: The study was done in Biochemistry department, Islamic International Medical
College,Rawalpindi and NIH.Islamabad.
Materials and Methods: Single injection of STZ was given intraperitoneally to rats and those rats that showed
fasting blood glucose level over 280mg/dl were included in the study. After induction of diabetes all rats were
divided into, normal control group (A), diabetes positive control group (B), and the two groups (C and D) served
as experimental groups while group E served as standard as it received glibenclamide. Group C and D diabetic
experimental rats received ethanolic extract of Cassia fistula leaves at 400 mg/kg and 500mg/kg of body weight
orally for eight weeks on daily basis. On the other hand group E rats received glibenclamide at 0.5 mg/kg body
weight orally for eight weeks. Blood samples were collected after eight weeks to find reno-protection against STZ
induced diabetic nephropathy.
Results: The diabetic positive group rats showed variable increase in levels of serum glucose, serum urea,
serum creatinine, total urinary protein and microalbuminuric levels. Body weight decreased and urine volume
increased in diabetic groups. Cassia fistula ethanolic extract of 400mg/kg and 500mg/kg body weight dose and
glibenclamide significantly decreased the levels of these parameters in rats. On comparison Cassia fistula
ethanolic extract of 500mg/kg dose reduced levels of biochemical parameters more effectively than the
400mg/kg dose of Cassia fistula and glibenclamide. Cassia fistula constituents, especially polyphenols and
flavonoids have strong anti-oxidant activity which might be involved in reno-protection.
Conclusion: Cassia fistula ethanolic leaf extract showed reno-protection against STZ induced diabetic
nephropathy in rats.
Farhana Adnan, Soban Sadiq, Adnan Jehangir. (2011) The Reno-Protective Effect of Ethanolic Extract of Cassia Fistula (Amaltas) Leaves on Streptozotocin Induced Diabetic Nephropathy in Rats, Journal of Islamic International Medical College, Volume-6, Issue-1.
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