• Shahida Mangi

• Anwar Hussain Phulpoto

• Muneer Ahmed Qazi

• Majid Ali Maitlo

• Rafique Ahmed Channa

• Nisar Ahmed Kanhar

Abstract

The present study aimed at surveillance of multidrug-resistant E. coli strains in clinical specimens, at district Khairpur Mir’s, Pakistan. A total of 280 clinical samples of different origins were collected from the tertiary care hospitals of Khairpur and Sukkur cities of Pakistan. Eschrichia coli strains were separated and identified using regular microbiological techniques and molecular biography using the 16S rRNA sequence-based homology. Antimicrobial sensitivity was determined using Kirby-Bauer's disc-diffusion assay and penicillin zone-edge test. Overall, ninety nine (99) strains of E. coli were isolated with maximum from pus 46 (46.6%) followed by urine 40 (40.4%), HVS 7 (7%) and stool 6 (6%). The E. coli in gender wise prevalence, was 100% prevalent in HVS samples, followed by pus (52%), urine (40%) and stool (33%) in female where as it was 67% prevalent in stool followed by urine (60%) and pus (48%) in male patient. The results of antibiotic sensitivity profiling revealed that Moxifloxacin was observed as most effective (78%) against all strains of E. coli while rest of the tested antibiotics were ineffective. Phylogenetic correlation of amplified 16S rRNA gene sequence of E. coli isolate shared 99% similarity with E. coli strain AS15. Prevalence of multidrug-resistant pathogen E. coli in clinical specimens calls for timely control measures to reduce health care cost and increasing resistance.

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