Abstract
The study was conducted to isolate the nodule borne Rhizobium spp. and to assess their antagonism to S. rolfsii, a soil borne pathogenic fungus of sunflower, non-leguminous plant. The bacteria were isolated from cluster bean (Cyamopsis tetragonoloba (L.) Taub. plants growing at various locations of Khairpur District, Pakistan. The five strains were found antagonistic on Czapek-Dox agar medium poured dual culture plates to Sclerotium rolfsii fungus. Isolate S7 was found highly efficient to check S. Rolfsii colony with 21mm inhibition zone followed by S6 (18mm), S9 (17 mm), S1 (13 mm) and S8 (12mm). The strains S7 and S6 were highly efficient to check the S. rolfsii growth in soil when inoculated in pot soil. The sunflower plant biomass observed with higher frequencies in dual infested soil (Rhizobium isolate S7, S6) with S. Rolfsii than control treatments. Thus the isolates S7 and S6 were found to be highly antagonist to S. rolfsii.

Abdul Rasheed Jatoi, Aziz Ahmed Ujjan, Nadia Ramzan, Nayara Naureen, Rabia A. Memon, Mumtaz Saand, Saleem Shahzad. (2018) ANTAGONISTIC ACTIVITY OF RHIZOBIUM ISOLATES TO FUNGUS SCLEROTIUM ROLFSII SACC. CAUSING COLLAR ROT OF SUNFLOWER (HELIANTHUS ANNUUS L.)., , Volume 15, Issue 4.
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