The phenomenon of emitting visible light by an organism as a consequence of a natural chemical reaction that take place inside them is called Bioluminescence. Wide number of marine animals and microbes possess this extraordinary feature and it is reported to be the primary source of light in the dark ocean. In the current study, the luminescent bacterial strain Vibrio chagasii (DGU227) isolated from the coastal water of Arabian Sea. The strain DGU227 showed sharp luminescence in presence of Ampicillin. The four genes DABE of lux operon were identified through PCR and the order of genes confirmed through intergenic sets of primers i.e., DABE. This unique feature of luminescence makes the isolated luminescent strain a potentially advantageous reporter for performing various genetic engineering experiments.
